About this manual. This manual describes the operation of the ÄKTA™FPLC™ system. System description, system maintenance and trouble-shooting are also. filter. Sample pump. Buffer select valve (V6). Injection valve (V1). Reverse . Manual. Flowpath. B1 or B2. BufferValveB. Pump A. Pump B. Common inlet. A2. A1. About this manual This manual describes the operation of ÄKTA purifier: Evaluating the UV response Calculate the UV response ratios in the following.
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The separation unit of the chromatography system has two main modules which are stacked on each other.
When entering a new buffer substance with only apparent pKa known, a conversion to thermodynamic pKa has to be performed. The Instant Run window opens. Superloop 10 ml and Superloop 50 ml must not be used at pressures above 4 MPa manyal bar, psi. Ionic strength Buffer components with several pKa values will give a higher ionic strength at the start when the pH is set above the second pKa and even higher above the third pKa for acidic buffer components.
This regulator tunes the actual pump flow rate to the set flow rate. You can choose to save the column globally i. If a lower detection sensitivity is desired due to output signal limitation, the 2 mm cell should be used.
Never place waste containers on top of the system. Scale-up to production is predictable and trouble-free.
The supply voltage for the components in the system and the fraction collector is distributed from the rear of the system. The color of a curve, its Y-scale, and its name are always the same. Adding information to the report 1 Click Edit Mode to enable changes in the report. The Page Setup dialog opens and you can, for example select page size and items to be included in the header and in the footer.
Peak fractionation is selected on the Elution Fractionation page in the Method Wizard. After a while the Windows desktop puririer.
ÄKTApurifier User Guide – GE Healthcare Life Sciences |
Up to 3 values pKa1- pKa3 can be entered for each buffering component. To set the default values, select System: Select the appropriate printer from the list and select Landscape. If the syringe is taken out before the sample is injected onto the column, self-drainage will occur and the loop will be emptied. Help us improve your experience by sending an error report. The parameters are found on the Peak Fractionation page.
ÄKTApurifier – GE Healthcare Life Sciences
The stirrer bar should rotate when the system is in Run mode. Fraction marks and fraction numbers make it easy to identify fractions and peaks. The mains kanual fuse is located to the right of the mains input. NaCl or KCl for elution gradient formation.
This allows for binary gradients with high pressure mixing. Release the mouse button. Run Data The Run Data pane displays the current values for selected run parameters. Manually select pump flow 0. If you manually alter the default values of the column, and thereby exceed the recommended values for the selected column, you will get a warning when you save your method.
PressLevel is the maximum allowed pressure. Find the thermodynamic pKa by taking the apparent pKa and add the value, found in the table below, for the buffer concentration at which the apparent pKa has been measured.
The main operating menu 1 of Monitor UPC shows the absorbance value with 6 digits for the selected wavelength, the conductivity as a percentage of full scale and the pH value optional. Follow the step-by-step instructions below and check the “Installation Record.
Unpack the inlet tubing contained in a plastic bag taped to the buffer tray on top of Box On delivery, the system is equipped with i.